| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders
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| Autores: |
Sastre,Darío A.
Argaraña,Carlos E.
Heller,Viviana B.
Gallo,Mónica
Fernández,Enrique N.
Rodríguez,Cecilia M.
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| Data: |
2007-01-01
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| Ano: |
2007
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| Palavras-chave: |
Multiplex-PCR
Ph chromosome
BCR-ABL
Chronic myeloid leukemia
PCR
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| Resumo: |
In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for e1a2, while in the BCR-ABL(+)AML patients a b3a2 rearrangement was observed. In conclusion, multiplex-PCR allows rapid, specific and simultaneous detection of different types of BCR-ABL transcripts in CML and ABL-BCR(+)AL. A full correlation was observed when multiplex-PCR was compared with nested PCR.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572007000400003
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.30 n.3 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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